Generator
Part:BBa_K105017:Design
Designed by: Michael Wild, Katja Karstens Group: iGEM08_ESBS-Strasbourg (2008-10-22)
Ura3 - Orotidine-5-phosphate decarboxylase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 210
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 210
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 210
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 210
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 900
Illegal SapI.rc site found at 747
Design Notes
Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design. So there is no base in between the BioBrick and the restriction sites. <br\n>
The deletation of an internal Pst1 site would be possible with a mutation in the non coding region of the gene, but hasn't been done yet. So for the moment this part can be only used as frontinsert in the last step of assembly.
Source
Amplification from the plasmid pRS416 (Sikorski & Hieter)