Generator

Part:BBa_K105017:Design

Designed by: Michael Wild, Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-22)

Ura3 - Orotidine-5-phosphate decarboxylase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 210
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 210
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 210
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 900
    Illegal SapI.rc site found at 747


Design Notes

Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design. So there is no base in between the BioBrick and the restriction sites. <br\n>

The deletation of an internal Pst1 site would be possible with a mutation in the non coding region of the gene, but hasn't been done yet. So for the moment this part can be only used as frontinsert in the last step of assembly.

Source

Amplification from the plasmid pRS416 (Sikorski & Hieter)


References